RNA

Part:BBa_K3328052

Designed by: Qi Wu   Group: iGEM20_OUC-China   (2020-10-03)


triggerB of NIMPLY gate (NIMPLY2)

NIMPLY2 triggerB

Design

In the NIMPLY gate, a deactivating RNA (INPUT A) uses direct hybridization or strand displacement to abolish trigger RNA (input B) activity. So when only the correct trigger RNA is expressed, the switch can be turned on.

799px-T--OUC-China--design_lunbo_nimply.jpg

Result

800px-T--OUC-China--part_NIMPLY2.jpg

The left figure shows that when INPUT A=0, INPUT B=1, the fluorescence of GFP is high. And the fluorescence intensity of GFP was low in the other three groups. This corresponds to the situation described in the truth table on the right. INPUT A=1 means that aTc (0.25 mg/mL) is added, INPUT B=1 means that HSL (0.1 mg/mL) is added. Error bar: SD (n=9).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 17
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 14
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 17
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 17
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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